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SOLVED: Moving nucleosomes out of the way is important for turning genes on. In yeast, the 11-subunit SWI/SNF complex, which is the founding member of the ATP-dependent chromatin remodeling complexes, is required for both activating and repressing gene transcription. How does it work? In principle, it could slide nucleosomes along the chromosome, knock them off the DNA, or transfer them from one duplex to another.To investigate this problem, you assemble a nucleosome on a 189-bp segment of label
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