13. The reaction catalyzed by pyruvate carboxylase includes which unstable intermediate? Which coenzyme required for this reaction? How is ATP hydrolysis coupled to the carboxylation of pyruvate? pyruvate HCO3 ATP = oxaloacetate ADP P
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SOLVED:Comment on the fact that the reduction of pyruvate to lactate, catalyzed by lactate dehydrogenase, is strongly exergonic (recall this from Chapter 15 ), even though the standard free-energy change for the half reaction Pyruvate 2 H^ 2 e^-→ Lactate is positive (ΔG^∘'=36.2 kJ mol^-1=8.8 kcal mol^-1), indicating anendergonic reaction.
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